Lab Resources

PLASMIDS

Please note that we are unable to handle the number of requests that we receive for plasmids and therefore cannot send plasmids directly from our lab. All of our published plasmids (see below) are available from the non-profit organisation, AddGene. If you deposit your own plasmids with AddGene you will accrue points that allow you to request plasmids free-of-charge. Otherwise, there is an administrative fee to pay for shipping, handling, maintenance and quality assurance:


Plasmids from: Lawrenson T, Shorinola O, Stacey N, Liu C, Østergaard L, Patron NJ, Uauy C, Harwood, W (2015) Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease Genome Biology 2015, 16:258

#68262    pICSL90003
#68261    pICSL90002
#68257    pICSL12009
#50270    pICSL12006
#68260    pICSL80037
#68259    pICSL80036
#50339    (from the Kamoun lab)
#46966    (from the Kamoun lab)
#68263    pICSL11059
#68252    pICSL11055
#68264    pICSL11060
#68258    pICSL11056

Level 0: PROM U6 (Triticum aestivum)
Level 0: PROM U6-26 (Arabidopsis thaliana)
Level 0: PROM Ubi (Zea mays)
Level 0: PROM CsVMV (Cassava Vein Mosaic Virus)
Level 0: CDS neomycin phosphotransferase II (Escherichia coli)
Level 0: CDS hygromycin phosphotransferase II (Escherichia coli)
Level 0: CDS Cas9 (Streptococcus pyogenes)
Level 0: sgRNA scaffold
Level 1, Position 1: CaMV35s:TMV-Omega_hptII with intron_CaMV35s
Level 1: CaMV 35S_nptII_nos
Level 1, Position 2: CsVMV_Cas9_CaMV35s
Level 1, Position 2: ZmUbi_Cas9_CaMV35s

Plasmids from: Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farré G, Rogers C, Smith A, Hibberd J, Webb AAR, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JDG, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, Martin C, Field R, O’Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loque D, Granell A, Tissier A, Shih P, Brutnell TP, Quick PW, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ané JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JAH, Ntoukakis V, Schäfer P, Denby K, Edwards KJ, Osbourn A, Jim Haseloff J (2015) Standards for Plant Synthetic Biology: A Common Syntax for Exchange of DNA Parts New Phytologist 208(1):13-9 

The Universal Acceptor Plasmid, pUAP1
63674             pUAP1


Plasmids from: Engler C, Youles M, Grützner R, Ehnert T-M, Werner S, Jones JDG, Patron NJ, Marillonnet S (2014) A Golden Gate Modular Cloning Toolbox for Plants ACS Synthetic Biology

The Golden Gate MoClo Plant Tool Kit (95 plasmid kit) Plasmids can also be ordered individually from this page.
The Golden Gate MoClo Plant Parts Kit (95 plasmid kit) Plasmids can also be ordered individually from this page.