Frequently used laboratory protocols:
We use a Type IIS parallel DNA assembly method (also known as Golden Gate Cloning). We have published a toolkit for plants as well as the PhytoBrick TypeIIS standard. Full details and a laboratory protocol can be found in this methods chapter: Patron, NJ (2016). DNA assembly for plant biology. Current Protocols in Plant Biology 1:604-616
To help new users get started with Type IIS parallel assembly, we made a training video that explains the background behind easy and reliable assembly of multiple standard parts in a single reaction and how to make your own standard parts. Some of the laboratory protocols are slightly out of date now, but it’s a good place to start understanding the background. You can also download a help-sheet on how to make new parts.